I don't believe filters allow excluding, only including.
So if you have 10 list items with Column1=A and 3 list items with Column2=B, you could filter to return only the items where Column1=A (thus excluding the 3 items with Column2=B).
However, you cannot filter (a default data connection) with a filter like Column2 is not equal to B.
One alternative might be to return ALL items in the list, and then filter the data on the canvas. A dropdown list box will allow a filter on the selection made in "Entries".
Good luck!